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Because the most commonly used drug tests often yield false negatives for Rohypnol, experts recommend use of gas chromatography-mass spectrometry analysis.

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The National Drug Intelligence Center (NDIC) says that in the United States GHB had surpassed Rohypnol as the substance most commonly used in DFSA, likely because GHB is much more easily available, cheaper and leaves the body more quickly.One new process can detect a 2 mg dose of Rohypnol for up to 28 days post-ingestion.Other tests for Rohypnol include blood and hair tests.After the drug wears off, users may find themselves unable to remember what happened while under its influence (anterograde amnesia), and feeling woozy, hung-over, confused, dizzy, sluggish and uncoordinated, often with an upset stomach.They may also have some difficulty moving their limbs normally.During social interactions alcohol consumption causes more biased appraisal of a partner’s sexual motives while impairing communication about and enhancing misperception of sexual intentions, effects exacerbated by peer influence about how to behave when drinking.

The Blade released a special report, "The Making of an Epidemic," criticizing a study conducted in the 1990s that concluded that 55% of rape victims had been intoxicated.

Most tests will detect benzodiazepines for a maximum of 72 hours after it was taken.

Most general benzodiazepine detection tests will not detect Rohypnol: the drug requires a test specifically designed for that purpose.

Mahmoud El Sohly of El Sohly Laboratories, test results indicated that flunitrazepam was only used in around 1% of reported date rapes according to Robertson and 0.33% according to urine lab tests done by El Sohly, of the rape-kits that actually get tested in time.

Despite having a long half-life (18–28 hours) an incorrect belief is that Rohypnol is undetectable 12 hours after administration which may result in victims failing to get a blood or urine test the following day.

There is currently no comprehensive data on how frequently DFSA occurs with the use of surreptitious drug administration.